If anyone can add to this table, please send your info to timcolton@aol. “Cop Logic,” allows anyone to conveniently submit a non-emergency incident from any internet-enabled PC or mobile device through this web-based online reporting system and immediately print a copy of. pastoris) and Saccharomyces cerevisae ( S. 1mW就是0dBm，小于1mW就是负数的dBm数。. The YDP-140 weighs 11 pounds more than the YDP-141. 2. The essential vitamins, minerals and amino. R200 Module Form FactorR200 ring flash, with or without grid. Lane M indicates a DNA size marker, 1 and 2 indicate passage 1 (P1), 3 and 4 indicate passage 5 (P5), and 5 and 6 indicate strain passage 10 (P10). R200-CPU40 Firmware. For more information about becoming a YPD Officer, contact: Officer Hailie Meyers, Hailie. cerevisiae S288C, FY1679-14C and BY4741 strains were grown in standard complex media, YPD (glucose 2%) from both suppliers, and the growth parameters were determined. Madelyn Kumar, Senior Photographer. Lots of other concentrations will be different. YPD medium. 17, two weeks after those colleagues alerted police. As a result of the crash, YPD responded to the area and closed off S. This medium is non-selective for Candida, Pichia, Saccharomyces, and Zygosaccharomyces. 5 subscribers. 5, 1 mM EDTA pH8. YPD (1 mL) was then added to cultivate the competent cells for 2 h, then the cells were washed twice with 1 mL sterile water, before plating the cells onto the corresponding SC medium. , the Yreka Police Department (YPD) says they received a call about a pedestrian who. 69. Using this protocol, we typically obtain ≥3 x 105 transformants per µg of plasmid DNA. Measure about 400ml Type 1 water and add to 1 liter bottle. 100 mm, 20 Plates per Sleeve, Sterile. 20 g. ondersteuning 860-960mhz of EU860-868mhz / US902-928mhz frequentieThis video explains RFID basics and walks you through the process of how to read and write RFID tags using a mobile handheld UHF RFID reader system. Dissolve the contents of the YP agar pouch from the PichiaPink™ Media Kit in 900 mL of distilled water. cerevisae) yeast strains. YPD-R200 RFID UHF module - Application board introductionShopping, Better Living! Aliexpress. Release. Patient and methods: A retrospective study of 118 pediatric KT performed between January 2007-July 2017. Check part details, parametric & specs updated 05 OCT 2022 and download pdf datasheet from datasheets. Indeed, the. 79. Frequency:865-868Mhz,902-928MHz for option Teknova YPD Agar Plates with Geneticin G418-200. 2 Relevant identified uses of the substance or mixture and uses advised against Identified uses : Laboratory chemicals, Synthesis of substances 1. LpJ8JXMR-Homicide-23-56323 Download. It utilizes a transmitter with a power of 100 mW, allowing for efficient and energy-saving operation within a radius of over 1. Yeast Growth on YPD Agar. Model No. On Monday night between 7:30 and 8:30 p. At the Yakima Police Department, we believe in building relationships—relationships between ourselves and the community we protect, relationships between our fellow officers and their families, and personal relationships to support us in what we do every day. m. It is preferred you. Getting Started with Digilent Pmod IPs. The following table summarizes the features. All Rights Reserved. 13. In this study, Xie et al. albicans strains on YPD were used to inoculate overnight cultures at 30°C. Introduction to RFIDIntroduction to RFID 7 Electronic Product Code (EPC) EPC is a product-numbering standard administered by GS1 that can be used to identify a variety of items 视频中讲解错误的地方，可用弹幕提出，方便大家学习理解。. YPD Police Chief Paulsen Retiring Paulsen Stepping Down After Eight Years At Yankton Helm. 012%, 0. Values are presented. Peptone. Yeast strains were stored in cryotubes with YPD (1% yeast extract, 2% peptone, and 2% glucose) and 15% glycerol at −80 °C. "Waste Ink Pads" installation (2) After checking "Waste Ink Tube". After the addition of DNA and treatment with DMSO, yeast cells are incubated in YPD Plus Liquid Medium—a formulation that enhances the uptake of plasmid DNA. 5 M glycine. 2. In the morning, check the OD600. Yamaha portatone (29 pages) Musical Instrument Yamaha PSR-S550 Owner's Manual. Yeast lipid content at 24 h of fermentation in Y. 0:04. It is a medium used for molecular cloning, protein expression, or as enrichment media. net Jun 18, 2018 Jun 18, 2018 Updated Nov 4, 2018; 0;Yamaha YP-D71. $71. The Maine medical examiner will conduct an investigation to determine a cause of death, YPD added. 77 mg/L resveratrol had been produced in YPD medium in a shake flask; this amount was 383. The internal buffer of the JRD-100 can hold up to 200 units, and it has the ability to read up to 50 radio tags per. nielsen@yankton. coli enzyme) are present in all SlCPTs, except for SlCPT3, which contains an L residue in place of E. After submitting your CV and application, you will be asked to participate in a video interview where you will answer a question related to your motivation for pursuing sustainability. 399801. (KYMA, KECY) - Yuma police have just identified the man who died in a motorcycle crash. YPD-200C Type Tablet Hardness tester is suitable to making trial of tablet's hardness in each of medicine factories, education and research of medicine, and medicine examining departments; Equipped with advanced transducer's circuit installation of taking in and taking off with motor for automatic measure, operation convenient, measuring accuracy high and good intuition of measuring value. Yeast cells were grown in YPD medium (2% glucose, 1% yeast extract, and 2% bactopeptone) at 30 °C with shaking. papatom Posts: 137. Origin. 1リットルあたりの組成：. Yamla Pagla Deewana also known as YPD is a series of Indian action comedy films, produced by Vijayta Films. albicans cells after 1 h of exposure to the diterpene. 1. download release notes. For YPD plates with drugs, prepare liquid medium as above, but add Bacto Agar (20 g/L) before autoclaving. Captain Shawn Boyle, - who is now the head of CID - said witnesses have been unwilling to testify. . According to a press release from the Yankton Police Department (YPD), a. Kenneth Strickland and Ms. Once completed, you may email the police department, mail the form, or drop off the form at the Yukon Police Department. $107. S 200. Aspergillus fumigatus is a major fungal pathogen causing life threatening infections in immunocompromised humans and certain animals. Store Box 2 at room temperature. This year, the event took place at Yale’s West Campus Conference Center and included the Yale Police Department’s (YPD) assistant chiefs, captains, lieutenants, and. com-专业的综合网上购物商城，为您提供正品低价的购物选择、优质便捷的服务体验。商品来自全球数十万品牌商家，囊括家电、手机、电脑、服装、居家、母婴、美妆、个护、食品、生鲜等丰富品类，满足各种购物需求。1. 06 Unauthorized use of a vehicle in the second degree 165. IMPORTANT: Verify that the medium has a pH of 6. As far as the best digital pianos with weighted keys go, it’s inevitable that Yamaha would feature on our list. To use the R200, you must also have a Godox AD200 or AD200Pro, which cost $299 and $349, respectively. The R200 isn’t just competing against other sub-$1000 bookshelf speakers; I think its performance is fully comparable to top-of-the-line $2,000-ish speakers like the KEF R3 and JBL HDI-1600. He served as an officer at the federal then local level for 30 years with the YPD. Originally written to read race timing chips on a finish line, a range of hardware is now supported from small desktop USB devices to long-range fixed readers, including the following, CF-RU5102 desktop UHF reader/writer (not HID version) The LilyPad Arduino family of boards has been designed for wearable applications. e. The components of this media provide all the necessary nutrients for yeast growth. gov, 509-575-3559 or HR Specialist Abbi Rhoads, Abigail. C. Many compete for YPD jobs. Cell density was estimated by colony counting, and proportionality constants k. This medium is non-selective for Candida, Pichia, Saccharomyces, and Zygosaccharomyces. Davis praised the officers for the work they have done while short-handed promising that “help is on the way. Rich Plating Media with Agar 1. 8 YPD also offers ongoing, specialized in-service training on topics such as community policing, bicycle patrols, and active shooter response. Check stock and pricing, view product specifications, and order online. Chafon RFID reader. The expression of V5-tagged Crd1p was inducedInside our R200, you can enjoy low-impact aquatic exercise, family fun, and swimming with our optional tether. Article Topic Follows: Local News. Objectives: Receiving a timely and accurate diagnosis and gaining access to age-appropriate support for younger people living with dementia (YPD) remains a challenge both in the UK and internationally because the focus of most dementia services is primarily upon the needs of older people. 100 mm, 20 Plates per Sleeve, Sterile. YPD agar is a complete yeast culture medium for most yeast species, especially for Saccharomyces cerevisiae. Programmable Logic. rhoads@yakimawa. The board I bought uses a serial interface, so it's pretty easy to control from an ESP32/Arduino. Ex: P-115B. Having used free-based cocaine/paste even one time within the past five years. The internal memory of the YDP-141 was increased from 337KB in the YDP-140 to 845KB. YUMA, Ariz. Guns Tip Hotline ($500 Reward): 377-GUNS. net arduino-rfid-R200. YPD medium, including 20 g/L glucose, 10 g/L yeast extract and 20 g/L tryptone was used for normal growth. 超高频uhf rfid r200系列远距离一体化读写器开发测试 概述 r200系列远距离一体化读写器为一体化设计的uhf 频段读写器，其具有多协议兼容、读取速率快、多标签识读、防水型外观设计等优点，可广泛的应用于各种rfid 系统中。 广泛应用于仓储管理、供应链管理. * Designated stand and. R200 1 poort UHF RFID lange afstand 1-20m passieve reader module met development board 2. The plates. 0. We would like to show you a description here but the site won’t allow us. org. No weighing of individual supplements, combining, or pH adjustment is required. 3 Meaning of A. ondersteuning 860-960mhz of EU860-868mhz / US902-928mhz frequentieExplore a wide range of the best YPD R200 on AliExpress to find one that suits you! Besides good quality brands, you’ll also find plenty of discounts when you shop for YPD R200 during big sales. 0. Order TE Connectivity Laird YPD1502P (YPD1502P-ND) at DigiKey. 嵌入式之RFID开发与应用 通俗易懂 难得教程 强烈推荐共计16条视频，包括：1. Headphones can be connected to the connection labeled “PHONE” or “PHONES/OUTPUT” located to the left of the instrument, underneath the keybed. Finally, the engineered strain produced a maximum titer of 125. YPD's top suspects for November 6, 2020. The assessment was conducted by 21CP Solutions, an external consulting firm that grew from former. Add 'FieldName' to your dashboard as a quick filter. Maximum specific growth rates. 2 R200 Camera Module The R200 module form factor option is the final product form factor for system integrators. The objective is to have healthy, log-phase cells in the morning. 台鐵R200型柴電機車 ，是臺灣鐵路管理局委託施泰德鐵路製造之幹線用大型柴電機車。 該款車型是臺鐵首款購入之歐系高性能柴油機車 。 該款車型為施泰德鐵路首度獲得來自臺鐵之訂單，也是該公司在亞太地區首筆訂單 。. 1 - Este Regulamento estabelece princpios e normas para a aplicao do Decreto-lei n 667, de 02 de julho de 1969, modificado pelo Decreto-lei n 1. Three Yakima Police Officers have been placed on administrative leave following an officer-involved shooting on the morning of May 3 that left one person dead according to the YPD. The pH of YPD medium was adjusted by L-malic acid to 6. Identificación de la sustancia Agar YPD para la biología molecular Número de artículo X971 Número de registro (REACH) no pertinente (mezcla) 1. Jones were Mr. Un radar est un système qui utilise des ondes radio pour détecter la présence, la distance et la vitesse d’objets à proximité. 20 g. 80 节省：￥59. 0, 4. Some of the YPD presidents to serve with Mrs. YUMA, Ariz. Store for several weeks at room temperature. YORK, Maine — Police offered praise and thanks to a tow truck driver whose shouted warning allowed a patrolman to narrowly escape being struck by a car drifting toward the side of Route 1. 2. If this is an emergency, call 911Non-emergency phone: 509-575-6200Monday-Friday 8:00am-5:00pm (Excluding Federally Recognized Holidays) The Yakima Police Department’s Online Police Reporting System allows anyone to conveniently submit a non-emergency incident from any internet-enabled PC or mobile device through this web. , click the "Chat Now" button on the website at havennh. ,Ltd. Jones instituted the February Black History Program which has become one of the high points for the YPD Conference Year. 1 and incubated shaking at 30°C until an OD 600 of 1. Suspend 65g of the product (Product No. 7 g/L yeast nitrogen base (without amino acids), 20 g/L. The H. Earline Hayes was elected YPD Director and served until 1991. Model Number: YPD-R200; Brand Name: YANPODO; Origin: Mainland China; Certification: CE; Dimension: Module:23*20*0. Install differential. Arduino R200 mikroprocesorowy czytnik RFID dalekiego zasięgu UHF moduł Raspberry Pi TTL UART bezpłatny Android SDK dla wbudowanego systemu 1, Mały uhf rfid Moduł czytnika kompatybilny z wieloma antena uhf rfid 0-12dbi. Due to weight and dimension restrictions, please request a shipping quote. Yeast extract–peptone–dextrose (YPD) medium: 10 g/L yeast extract 10 g/L, 20 g/L Bacto peptone 20 g/L, 20 g/L glucose, 100 mg/L adenine hemisulfate. It works on rechargeable batteries and allows easy connection with sensors and actuators developed for an easy integration in clothes and fabrics. (KYMA, KECY) - The Yuma Police Department (YPD) sent out a warning regarding a certain phone scam to locals. Use a sterile loop to inoculate a 50 ml tube containing 5 ml YPD medium with the INVSc1 yeast stab. 3. "Unlawfully" means in violation of article thirty-three of the public health law. The tonearm seems like a simplified preview of the ones built for the very successful GT-750 / GT-1000 / GT-2000. The internal buffer of the JRD-100 can hold up to 200 units, and it has the ability to read up to 50 radio tags per. 4. The ρ 0 derivatives of the strains described above were obtained by plating the cells twice on YPD agar plates (2% glucose, 1% yeast extract, 2% bactopeptone, and 2% agar) containing 40 μg/ml ethidium bromide . ERICSON BENJAMIM MELO DE ARRUDA. The blue color of the solution is due to the presence of copper and the copper-chelated form of Zeocin. If you or anyone have information about this case, call YPD at 928-783-4421 or 78-Crime at (928) 782-7463 to remain anonymous. Pike Pole Fiberglass 15 Inch. Approach to Policing. YUMA, Ariz. For comparative analysis of opai transcript and protein levels, five representative transformants with an average CLA yield in each series were selected, along with the best-performing. Invelion R200 Chip Micro Size Long Range Rfid Reader Uhf Module Raspberry Pi Ttl Uart Free Android Sdk For Embedded System , Find Complete Details about Invelion R200 Chip Micro Size Long Range Rfid Reader Uhf Module Raspberry Pi Ttl Uart Free Android Sdk For Embedded System,Raspberry Pi Ttl Uart,Free Android Sdk For Embedded System,Rfid. Application process and selection criteria. Ex: CVP-709. Add Zeocin from a 100 mg/mL stock to a final concentration of 100 µg/mL, 500 µg/mL, or 1000 µg/mL as needed. A woman from Yreka has been charged with murder after a hit-and-run. prospect, yl)silanti, mi 48198 august 15, 2016 7:00 p. The next day culture was diluted in YPD medium to grow at 30 °C for at least two generations to A 600 = 4–8. Henne and Grow were among the YPD officers who went to the store after a witness spotted a Chevrolet Colorado pickup truck that had been reported stolen in a Wapato robbery, according to court. Formulario De Quejas De Personal. Ship status is indicated as either currently active [A] (including ready reserve), inactive [I], or precommissioning [P]. Roads were temporarily closed but have since reopened. If you need non-emergency police response, please call (734) 994-2911 to reach central dispatch. Keep it tuned to our newscasts. A complete set of self-paced training. Now set the quick filter to show 'Only Relevant Values'. With the YDP-140, users only have the option of recording one track at a time. The protest, which took place outside the. Conventional complex media are routinely used to grow auxotrophic strains under the assumption that they can compensate the latter’s nutritional deficiencies. YPD Protocol. Descriptions. Here, we describe a method for affinity maturation of engineered antibody domains (eAds) using yeast display. th. Sewell, the Commissioner of the New York City Police Department (“NYPD”), and Christopher Sapienza, Commissioner of the City of Yonkers Police Department (“YPD. Sajena Clay, Episcopal YPD Director Mr. The essential vitamins, minerals and amino. Read and write RFID tags using the popular Chafon UHF-based readers and writers. ID Number. Add 2 × 10 8 cells to 4 L of freshly made 1× YPD (autoclaved for 20 min). Smarter Shopping, Better Living! 0. Saturday between Seventh and Naches. com • EN-CA-1014-1 Transmission 2X 3. (B) YPD plating gives a more accurate higher viability ofSecond, the five residues implicated in substrate binding (F70, S71, R194, R200 and E213; amino acid numbers corresponding to the E. Eric Wang. Store for several weeks at room temperature. ondersteuning 860-960mhz of EU860-868mhz / US902-928mhz frequentie Explore a wide range of the best YPD R200 on AliExpress to find one that suits you! Besides good quality brands, you’ll also find plenty of discounts when you shop for YPD R200 during big sales. The ΔsskA ypd1tet−on, but not the ΔsskAΔskn7 ypd. 24 /unit (ex. Take differential in box to Unitrax in Anaheim. 4–0. Read range: 1-20m(depend on tag, antenna and application) Power supply: 3. Accessories and Attachments. Store autoclaved medium at room temperature in subdued light. [3] And the third part named Yamla Pagla Deewana: Phir Se was released on 31 August 2018. In a press release, the incident occurred on Thursday at 8:45pm when YPD received a report about a verbal threat made by a student towards a teacher and was in possession of a BB gun. Standard curve’s reliability along time. Scott is a career law enforcement officer, attorney and 30-year veteran who has dedicated his life to public service. Impinj's IPJ-R2000 is a nfc/rfid read/write 840mhz to 960mhz 64-pin qfn ep. 20 g デキストロース（グルコース）. Dimension: Module:23*20*0. This notion is based onIf this is an emergency, call 911Non-emergency phone: 509-575-6200Monday-Friday 8:00am-5:00pm (Excluding Federally Recognized Holidays) The Yakima Police Department’s Online Police Reporting System allows anyone to conveniently submit a non-emergency incident from any internet-enabled PC or mobile device through this web. After declaring that the fire was intentional, YFD called YPD's Investigation Unit to assist them. Fuel injection unit is an essential mechanism of diesel engines, and thus, has to be designed to allow fine adjustment to the engine load. M. Download and run directly onto the system you want to update. 06 Gang assault in the second. I am an applicant who wishes to and has requested permission from the Yakima Police Department ("YPD") to ride in a Yakima Police vehicle (referred to in this document as a "police vehicle"). 2 Relevant identified uses of the substance or mixture and uses advised against Identified uses : Laboratory chemicals, Synthesis of substances 1. 5M stocks(pH6–7)that were filter-sterilized. A small group of students were sitting in the Founder’s Room inside The House when the incident occurred on Nov. R200 1 poort UHF RFID lange afstand 1-20m passieve reader module met development board 2. Abstract. 2, Z dwiema częstotliwościami 902-928mhz (częstotliwość usa) i 865-868mhz (ue Częstotliwość) YPD said there were reports of shots being fired in the parking lot and the suspect left the scene on foot and a vehicle could have been involved. Spot. The addition of protein and yeast cell extract hydrolysates. Getting Started with Digilent Pmod IPs. YPD’s formulation is the same but without the additional adenine. The cultures were diluted in fresh YPD to an OD 600 of 0. Both the. The formal promotions of YPD Officer Scott Grant and YPD Corrections Officer Jason Masters will take place during a. The components of this media provide all the necessary nutrients for yeast growth. Rare LP record turntable of the superlative yet easy-to-use kind : a bigger YP-D8. The shelf lifefor YPD medium is several months. The HOG pathway is for two reasons interesting in this context: Firstly, it is a stress signaling pathway that contributes to the ability of this. Yanpodo UHF RFID โมดูล Arduino Board 1-15M Long Range RFID โมดูล UHF Reader Raspberry Pi Android USB TTL UART SDK ฟรีThe cultures were all diluted to 0. 02%, 0. $46. lipolytica has significant advantages regarding the microbial synthesis of resveratrol. 2 g/l (equivalent to 19. 18 volt 2-speed drill-driver (36 pages) Drill Ryobi P202 Operator's Manual. Cultures were washed, adjusted to an initial OD 750nm of 0. For the preparation of solid medium, 2% (w/v) agar was added into liquid LB and YPD. 现价：￥190. 规格：50 ml. The cells were harvested by centrifugation at 3000 rpm for 5 min, washed with equal. In rare instances, the New Haven Police will have jurisdiction; the YPD will still remain a resource and will manage an. factory direct supply, meest scherpe prijs 3. YPD 培养基 (YPD Plus ) 分类：微生物培养. Every January, Chief Ronnell Higgins gets his leadership team together for an offsite retreat. 2. 6MB] PSR-E203/YPT-200 Owner's Manual (Text Version) — [75KB]Dockside Physiotherapy, Victoria, British Columbia. 5 to 0. It does not mean that the church was founded in Africa or that it is for people of African descent only. built-in stroboscope. Peptic digest of animal tissue provides nitrogenous nutrients. Peptic digest of animal tissue provides nitrogenous nutrients. Staff Reporters. Damian Williams, the United States Attorney for the Southern District of New York, Michael J. SKU. ” But Youngstown also has to deal with what is perceived as a low starting salary. It is known that the PD-L1 antibody binds to PD-L1 on the cell surface and leads to subsequent PD-L1 internalization 20. When it's time to party, invite your friends, because this swim spa comfortably fits up to 10 adults. “It was all I ever wanted,” he remembered. An initiative of former YPD Chief James Perrotti, the police academy began after the YPD relocated five years ago from its old headquarters on Sachem Street to the Rose Center in the Dixwell neighborhood, Higgins said. m. 000$ YPD Tournaments at GGPoker * More information here *. Cross Ref: 2LTA3 / YPD-6. Yonkers Police Records Division, 104 South Broadway, 2nd Floor, Cacace Justice Center. Portsmouth Herald. 最適化された. We are starting with the Yamaha Clavinova CLP-735, a modern piano with. 2. One of Yamaha’s finest. You can also contact the YPD directly at 203-432-4400. Amino acid powder mix: Mix 6 g of each of the amino acids listed in Table 1, supplement the mix with 6 g of adenine hemisulfate. 4. This is how Tony D sets up his R200 and R180 differentials: Find large box. 博通已成为国内 消费电子 和 工业 应用无线IC的市场. Support:GPS,4G,WIFI,Bluetooth,SIM 3. See variants (10). Les radars sont largement utilisés dans de nombreuses applications, notamment la navigation aérienne, la surveillance de la circulation routière. Police Emergency Number (Cell Phone): 377-7777. Diecast Zinc armbase. For research use only. The crosslinking was performed in 1% formaldehyde and quenched by adding 10 ml of 2. The Yakima Police Department (YPD) will celebrate the promotion of two of its employees, one a police officer and the other a corrections officer, to the rank of sergeant during a ceremony on Thursday, September 5 th. 100 and has been provided on Nov 22, 2023 a 4:12pm 25 inmates found. Kaaz, Cusco, Tomei, Os Giken (Drooool, os giken), Carbonetics, Quaife (More droolage), Obx- The options are ridiculously endless. Product name : YPD Agar Product Number : Y1500 Brand : Sigma-Aldrich 1. 答：其实归根到底为什么接收的无线信号是负值。. csdn. The plates. It is always a mutation in a single gene; the arginine permease. 2. Servo-controlled. What are the YPD Divisions Structure? Mother Sunbeams, 2-6 years old; Allen Stars, 7-12 years old; Youth Auxiliary 13-17 years old; and Young Adults, 18-26 years old. Historically, the effectiveness of a police agency was measured in terms of response time. In analogy to the results presented above. coli strains were cultured in 100 μg/mL ampicillin LB broth or agar plate. *For use with systems running Windows® 11 / Windows® 10 64-bit version 1809 and later. 0 3 Reviews ౹ 25 sold Color: 100pcs. 5 B) reached a wet cell weight of 93. The hole type/pintle type of fuel injection nozzle are used according to the engine type. Dissolve reagents in 1 L of distilled water. m. "We've. Julia Edinger. 读写器天线端口接50 (负载或用高频电缆连接天线。. Monday thru Friday, 9:00 AM to 3:00 PM. List of Components Store Box 1 at –20° C. gov. The political case to improve services for YPD depends upon. Ronnell Higgins, associate vice president for public safety and community engagement and former Yale Chief of Police, has been appointed to succeed James Rovella as the Commissioner of the Connecticut. 8% higher than that produced by strain YAr4. 5, 3. 11/07/2023 09:59AM. E. curvatum NRRL-Y-1511 grown in YPD and VFAs-synthetic media was determined gravimetrically and by fluorescence to build different standard curves with 6-month difference.